Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-G3BP-1 antibody. The HRP-conjugated Goat anti-Mouse IgG (H + L) antibody was used to detect the antibody.
Synthesized peptide derived from human protein. AA range: 200-300.
Key Feature
Clonality
Monoclonal
Isotype
IgG2b,k
Host Species
Mouse
Tested Applications
WB
WB:1:500-1:2000
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Protein G
Target Information
Gene Symbol
G3BP1
Gene Synonyms
G3BP HDH-VIII
Gene Full Name
G3BP stress granule assembly factor 1
Gene Summary
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Alternative Names
Ras GTPase-activating protein-binding protein 1
G3BP-1
EC 3.6.4.13
ATP-dependent DNA helicase VIII
hDH VIII
GAP SH3 domain-binding protein 1
Molecular Weight(MW)
52 kDa(Observed band size: 68 kDa)
Cellular Localization
Cytoplasm,Nucleus.
Application
WB
Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-G3BP-1 antibody. The HRP-conjugated Goat anti-Mouse IgG (H + L) antibody was used to detect the antibody.