WB
Western blot analysis of phospho-NLRC4(Ser-533) on NIH/3T3 cells lysates using anti- phospho-NLRC4(Ser-533) antibody at 1/1,000 dilution.ICC/IF
Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.| Product Name | Phospho-NLRC4 (S533) Rat Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide of mouse phospho-NLRC4(Ser-533) (AA: 525-538) expressed in E. Coli. |
| Modification | p-S533 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Rat |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NLRC4 |
|---|---|
| Gene Synonyms | CLAN IPAF AIFEC CLAN1 CLANA CLANB CLANC CLAND FCAS4 CARD12 CLR2.1 |
| Gene Full Name | NLR family CARD domain containing 4 |
| Gene Summary | This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| Alternative Names | and NACHT-containing protein antibody CARD 12 antibody CARD antibody CARD LRR and NACHT containing protein 1 antibody CARD LRR and NACHT containing protein antibody CARD12 antibody Caspase recruitment domain containing protein 12 antibody Caspase recruitment domain family member 12 antibody Caspase recruitment domain-containing protein 12 antibody CLAN 1 antibody CLAN A antibody CLAN antibody CLAN B antibody CLAN C antibody CLAN D antibody Clan protein antibody CLAN1 antibody CLANA antibody CLANB antibody CLANC antibody CLAND antibody CLR 2.1 antibody CLR2.1 antibody ICE protease activating factor antibody Ice protease-activating factor antibody Ipaf antibody LRR antibody NLR family CARD domain containing 4 antibody NLR family CARD domain-containing protein 4 antibody NLRC 4 antibody NLRC4 antibody NLRC4_HUMAN antibody NOD like receptor C4 antibody Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 4 antibody UNQ6189/PRO20215 antibody |
| Molecular Weight(MW) | 117 kDa |
| Cellular Localization | Cytoplasm. Cytoplasmic filaments. Nucleus. |
WB
Western blot analysis of phospho-NLRC4(Ser-533) on NIH/3T3 cells lysates using anti- phospho-NLRC4(Ser-533) antibody at 1/1,000 dilution.
ICC/IF
Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.| Application Notes | WB:1:500-1:2000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
