Application
Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 μg/mL.Application
Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 μg/mL.Application
Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/mL, respectively.| Product Name | Ipaf Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ipaf Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NFκ signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.1) Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89. |
| Immunogen | Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf. The immunogen is located within the last 50 amino acids of Ipaf. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 μg/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRC4 |
|---|---|
| Alternative Names | Ipaf Antibody: CLAN IPAF CLAN1 CLANA CLANB CLANC CLAND CARD12 CLR2.1 CLAN UNQ6189/PRO20215 NLR family CARD domain-containing protein 4 CARD LRR and NACHT-containing protein Clan protein |
| Molecular Weight(MW) | 110 kDa |
| Sequence Similarities | 3) Cat. No. 17-008 - THP-1 Cell Slide |
Application
Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 μg/mL.
Application
Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 μg/mL.
Application
Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/mL, respectively.| Positive Control | 1) Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate |
|---|---|
| Application Notes | Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 μg/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Ipaf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ipaf Antibody is supplied in PBS containing 0.02% sodium azide. |
