WB
Western blot analysis of SNAP29 on different lysates with Rabbit anti-SNAP29 antibody at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | SNAP29 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SNAP29 aa 1-258 / 258. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SNAP29 |
|---|---|
| Gene Synonyms | CEDNIK SNAP-29 |
| Gene Full Name | synaptosome associated protein 29 |
| Gene Summary | This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | CEDNIK antibody FLJ21051 antibody SNAP 29 antibody SNAP-29 antibody SNAP29 antibody SNP29_HUMAN antibody Soluble 29 kDa NSF attachment protein antibody Synaptosomal associated protein 29 antibody Synaptosomal associated protein 29kDa antibody Synaptosomal-associated protein 29 antibody Vesicle membrane fusion protein SNAP 29 antibody Vesicle membrane fusion protein SNAP29 antibody Vesicle-membrane fusion protein SNAP-29 antibody |
| Molecular Weight(MW) | 29 kDa |
| Cellular Localization | Cytoplasm, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Cell projection, cilium membrane. |
WB
Western blot analysis of SNAP29 on different lysates with Rabbit anti-SNAP29 antibody at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
