Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GST tag antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
The specific immunogen used to produce this antibody is proprietary information.
Key Feature
Clonality
Monoclonal
Isotype
IgG,K
Host Species
Rabbit
Tested Applications
WB
WB:1:50000-1:100000
Species Reactivity
All
Concentration
1mg/ml
Purification
Protein A
Target Information
Gene Symbol
GST tag
Function
GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells.
Application
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GST tag antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.