Application
Western blot analysis of extracted from transformed E.coli which expressing GST-tagged protein, using GST-tag antibody. A: Non-induction E. coli lysate B: Induction E. coli lysate 10% SDS PAGE diluted at 1:5000| Product Name | GST tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal antibody to GST tag |
| Immunogen | GST protein of the parasitic helminth Schistosoma japonicum expressed in E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G10 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| For WB: Use at a concentration of 0.5 - 2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | All |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | GST tag |
|---|---|
| Target | GST |
| Light Chain Type | kappa |
Application
Western blot analysis of extracted from transformed E.coli which expressing GST-tagged protein, using GST-tag antibody. A: Non-induction E. coli lysate B: Induction E. coli lysate 10% SDS PAGE diluted at 1:5000| Application Notes | For WB: Use at a concentration of 0.5 - 2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
