WB
25 ug total protein per lane of various lysates (see on figure) probed with A2M monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with A2M Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit) and DAB staining.| Product Name | A2M Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human alpha 2 Macroglobulin : 1200-1251/1474. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | A2M |
|---|---|
| Gene Synonyms | A2MD CPAMD5 FWP007 S863-7 |
| Gene Full Name | alpha-2-macroglobulin |
| Gene Summary | The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified. [provided by RefSeq, Nov 2016] |
| Alternative Names | A2MD CPAMD5 FWP007 S863-7 A2mp A2MAC1 A2m1 A2maa A2mb Mam A2MG_HUMAN A2M Alpha-2-M C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 PZP_MOUSE Pzp Alpha-2-macroglobulin (Alpha-2-M) A2MG_RAT alpha-2-macroglobulin |
| Molecular Weight(MW) | 160 kDa(Observed band size: 180 kDa) |
| Cellular Localization | Secreted. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with A2M monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with A2M Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit) and DAB staining.| Application Notes | WB:1:1000-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
