WB
Western blot analysis of extracts of various cell lines, using KMT2A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.ICC/IF
Immunofluorescence analysis of HeLa cells using KMT2A antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.| Product Name | KMT2A polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human KMT2A(NP_005924.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KMT2A |
|---|---|
| Gene Synonyms | HRX MLL ALL1 GAS7 HTRX MLL1 TRX1 ALL-1 CXXC7 HTRX1 MLL1A WDSTS |
| Gene Full Name | lysine methyltransferase 2A |
| Gene Summary | This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010] |
| Alternative Names | KMT2A ALL-1 CXXC7 HRX HTRX1 MLL MLL1 MLL1A TRX1 WDSTS MLL-AF9 MLL/GAS7 TET1-MLL |
| Molecular Weight(MW) | 190 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts of various cell lines, using KMT2A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of HeLa cells using KMT2A antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
