IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using KMT2A mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using KMT2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.FC
Flow cytometric analysis of Hela cells using KMT2A mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to KMT2A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human KMT2A (AA:150-400) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHC |
| IHC:1:200 - 1:1000 ICC/IF:1:200 - 1:1000 FC:1:200 - 1:400 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KMT2A |
|---|---|
| Gene Synonyms | HRX MLL ALL1 GAS7 HTRX MLL1 TRX1 ALL-1 CXXC7 HTRX1 MLL1A WDSTS |
| Gene Full Name | lysine methyltransferase 2A |
| Gene Summary | This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010] |
| Alternative Names | HRX MLL MLL1 TRX1 ALL-1 CXXC7 HTRX1 MLL1A WDSTS |
| Molecular Weight(MW) | 431 kDa |
| Cellular Localization | Nucleus. |
IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using KMT2A mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using KMT2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.
FC
Flow cytometric analysis of Hela cells using KMT2A mouse mAb (green) and negative control (red).| Application Notes | IHC:1:200 - 1:1000 ICC/IF:1:200 - 1:1000 FC:1:200 - 1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
