WB
Western blot analysis of extracts of various cell lines, using NOP58 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.ICC/IF
Confocal immunofluorescence analysis of U2OS cells using NOP58 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Product Name | NOP58 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human NOP58(NP_057018.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOP58 |
|---|---|
| Gene Synonyms | NOP5 HSPC120 NOP5/NOP58 |
| Gene Full Name | NOP58 ribonucleoprotein |
| Gene Summary | The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs. [provided by RefSeq, Nov 2015] |
| Alternative Names | NOP58 HSPC120 NOP5 NOP5/NOP58 |
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts of various cell lines, using NOP58 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.
ICC/IF
Confocal immunofluorescence analysis of U2OS cells using NOP58 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
