Application
Antibody recognizes a single 57kDa band in blots of whole yeast protein extracts.| Product Name | Nop5p + Nop58p antibody [34B12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [34B12] to Nop5p + Nop58p - Nucleolar MarkerNop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in S. cerevisiae (baker's yeast). |
| Immunogen | This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 34B12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). IP: Use 2-4 μl for the amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (ECL). Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Does not immunoprecipitate as well as another Nop5p monoclonal antibody, GTX24640. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Yeast |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | NOP5 |
|---|---|
| Target | Nop5p + Nop58p |
| Cellular Localization | Nuclear (nucleolus) |
Application
Antibody recognizes a single 57kDa band in blots of whole yeast protein extracts.| Application Notes | IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). IP: Use 2-4 μl for the amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (ECL). Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Does not immunoprecipitate as well as another Nop5p monoclonal antibody, GTX24640. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 10mM Sodium Azide; Constituents: Tissue Culture Supernatant |
