| Product Name | Nop5p + Nop6p antibody [37C12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [37C12] to Nop5p + Nop6p - Nucleolar Marker |
| Immunogen | This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 37C12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIP |
| IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). IP: Use 2-4 μl with the amount of lysate derived from 5 OD600 units of yeast culture. Does not work well for immunoblotting, for which we recommend GTX24639. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Yeast |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | NOP5 |
|---|---|
| Target | Nop5p + Nop6p |
| Cellular Localization | Nuclear (nucleolus) |
| Application Notes | IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). IP: Use 2-4 μl with the amount of lysate derived from 5 OD600 units of yeast culture. Does not work well for immunoblotting, for which we recommend GTX24639. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 10mM Sodium Azide; Constituents: Tissue Culture Supernatant |
