WB
The membrane was incubated with anti-4 Hydroxynonenal antibody at a dilution of 1:5000. The HRP-conjugated Goat anti-Mouse IgG (H + L) antibody was used to detect the antibody.
WB
BSA and 4-HNE-BSA were separated by 4-20% SDS-PAGE, and the membrane was incubated with anti-4 Hydroxynonenal antibody at a dilution of 1:5000. The HRP-conjugated Goat anti-Mouse IgG (H + L) antibody was used to detect the antibody.
IHC
Human kidney was stained with anti-4 Hydroxynonenal Mouse antibody.