WB
Western blot analysis of 4-Hydroxynonenal on different lysates with Mouse anti-4-Hydroxynonenal antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: 4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cardiac atherosclerosis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 4-HNE(0.2mg/ml 30min). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).| Product Name | 4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000 IHC:1:200-1:500 ICC/IF:1:500 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | (2E)-4-Hydroxy-2-nonenal 2-Nonenal 4-hydroxy- (2E 4R)- 4 HNE 4Hydroxynonenal 4-Hydroxynonenal 4-hydroxy-trans-2-nonenal HNE 4-HNE 4 Hydroxynonenal |
|---|---|
| Molecular Weight(MW) | 35-150 kDa |
| Function | 4-Hydroxynonenal, or 4-hydroxy-2E-nonenal or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an α,β-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary α,β-unsaturated hydroxyalkenal formed in this process. It is a colorless oil. It is found throughout animal tissues, and in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction, due to the increase in stress events. 4-HNE has been hypothesized to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion. |
WB
Western blot analysis of 4-Hydroxynonenal on different lysates with Mouse anti-4-Hydroxynonenal antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: 4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cardiac atherosclerosis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 4-HNE(0.2mg/ml 30min). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).| Application Notes | WB:1:5000 IHC:1:200-1:500 ICC/IF:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
