WB
Various lysates were subjected to SDS PAGE followed by western blot with NFATC2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using NFATC2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using NFATC2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).FC
1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human NFATC2 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | NFATC2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NFATC2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCIHCWB |
| WB:1:1000-1:4000 IHC :1:50-1:500 FC:0.20 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | NFATC2 |
|---|---|
| Gene Synonyms | JCOSL NFAT1 NFATP |
| Gene Full Name | nuclear factor of activated T cells 2 |
| Gene Summary | This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012] |
| Alternative Names | NFAT1 NFATP 1F3B2 NF ATc2 NF ATp |
| Molecular Weight(MW) | 100 kDa(Observed band size: 135-140 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with NFATC2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using NFATC2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using NFATC2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC
1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human NFATC2 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:1000-1:4000 IHC :1:50-1:500 FC:0.20 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
