WB
Recommended dilution: 500-2000. Gel: 12%SDS-PAGE. Lysate: 5μg. Lane 1-2: HEK293T cels lysates were transfected with the pCMV6-ENTRY control and pCMV6-ENTRY Dendra2 cDNA for 48 hrs. Primary antibody: Dendra2 Antibody at dilution 1/500. Secondary antibody: Goat anti mouse IgG at 1/8000 dilution. Exposure time: 2 minutes.| Product Name | Anti-Dendra2 mouse monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Dendra2 expressed in HEK293 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Dendra2 |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Molecular Weight(MW) | 28 kDa |
|---|---|
| Function | Dendra2 is a photoswitchable (green to red), monomeric fluorescent protein derived from octocoral Dendronephthya sp. The Dendra2 sequence has been optimized for maturation and a bright fluorescence both before and after photoswitching. Its codon usage is optimized for high expression in mammalian cells, but Dendra2 can be expressed in many other systems as well. |
WB
Recommended dilution: 500-2000. Gel: 12%SDS-PAGE. Lysate: 5μg. Lane 1-2: HEK293T cels lysates were transfected with the pCMV6-ENTRY control and pCMV6-ENTRY Dendra2 cDNA for 48 hrs. Primary antibody: Dendra2 Antibody at dilution 1/500. Secondary antibody: Goat anti mouse IgG at 1/8000 dilution. Exposure time: 2 minutes.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
