ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.IF-P
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using COL1A1 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:300 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Product Name | Anti-COL1A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIF-P |
| ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COL1A1 |
|---|---|
| Gene Synonyms | CAFYD Col1a-1 Cola-1 Cola1 COLIA1 EDSARTH1 EDSC Mov-13 Mov13 OI1 OI2 OI3 OI4 |
| Gene Full Name | collagen type I alpha 1 chain |
| Gene Summary | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Alternative Names | alpha 1 (I) chain propeptide alpha-1 collagen type 1 alpha-1 collagen type I alpha-1 type 1 collagen alpha-1 type I collagen alpha1(I) procollagen COL1A COL1A1 Collagen 1 collagen alpha 1 chain type I collagen alpha-1(I) chain preproprotein collagen Col1-ColIII-1 collagen Col1-ColIII-2 Collagen I collagen of skin tendon and bone alpha-1 chain collagen type 1 alpha 1 collagen type I alpha 1 Collagen1A1 pro-alpha-1 collagen type 1 pro-alpha-1 type I collagen procollagen type I alpha 1 procollagen type 1 alpha 1 procollagen type I alpha 1 Type I Collagen type I proalpha 1 type I procollagen alpha 1 chain type I-alpha 1 collagen |
| Molecular Weight(MW) | 138kDa |
| Source | Rabbit |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| SwissProt ID | P02452 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.
IF-P
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using COL1A1 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:300 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Application Notes | ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
