WB
WB analysis of human cervical cancer using Collagen Type I Monoclonal antibody. human cervical cancer tissue were subjected to SDS PAGE followed by western blot with Collagen Type I antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
WB analysis of pig lung using Collagen Type I Monoclonal antibody. pig lung tissue were subjected to SDS PAGE followed by western blot with Collagen Type I antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human breast cancer using Collagen Type I Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Collagen Type I antibody at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF Staining of Human Fibroblasts using Collagen Type I Monoclonal antibody. Human Fibroblasts cells were detected by western blot and Immunofluorescent analysis with Collagen Type I antibody. (Fig from Customer)IF-P
IF Staining of human colon cancer using Collagen Type I Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Product Name | Collagen Type I Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:5000-1:50000 IHC:1:2500-1:10000 ICC/IF:1:400-1:1600 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | COL1A1 |
|---|---|
| Gene Synonyms | OI1 OI2 OI3 OI4 EDSC CAFYD EDSARTH1 |
| Gene Full Name | collagen type I alpha 1 chain |
| Gene Summary | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Alternative Names | COL1A1 1E9A7 Alpha 1 type I collagen Alpha-1 type I collagen COL I |
| Molecular Weight(MW) | 139 kDa(Observed band size:100-140 kDa，200kDa) |
| Cellular Localization | Extracellular matrix,Secreted. |
WB
WB analysis of human cervical cancer using Collagen Type I Monoclonal antibody. human cervical cancer tissue were subjected to SDS PAGE followed by western blot with Collagen Type I antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
WB analysis of pig lung using Collagen Type I Monoclonal antibody. pig lung tissue were subjected to SDS PAGE followed by western blot with Collagen Type I antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human breast cancer using Collagen Type I Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Collagen Type I antibody at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF Staining of Human Fibroblasts using Collagen Type I Monoclonal antibody. Human Fibroblasts cells were detected by western blot and Immunofluorescent analysis with Collagen Type I antibody. (Fig from Customer)
IF-P
IF Staining of human colon cancer using Collagen Type I Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:5000-1:50000 IHC:1:2500-1:10000 ICC/IF:1:400-1:1600 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
