ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using glutamate metabotropic receptor 5 antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),U251(5),A549(6) . 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-glutamate metabotropic receptor 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GRM5 |
|---|---|
| Gene Synonyms | 6430542K11Rik Glu5R GPRC1E mGlu5 MGLUR5 mGluR5b PPP1R86 |
| Gene Full Name | glutamate metabotropic receptor 5 |
| Gene Summary | This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Alternative Names | A830007B11Rik D16Ium24 D16Ium24e G protein-coupled receptor GRM5 G protein-coupled receptor family C group 1 member E Glur-5 Glur5 Glurbeta1 glutamate receptor glutamate receptor metabotropic 5 GPCR family C GPRC1A GRM 1 GRM 5 GRM1 GRM1 alpha GRM1A metabotropic 1 metabotropic glutamate receptor (mGluR5) metabotropic glutamate receptor 5b metabotropic glutamate receptor subtype 5 homolog metabotropic glutamate receptor subtype 5b metabotropic glutamate receptor type 5 MGC25101 mGlu1 mGluR mGluR1 mGluR1 alpha mGluR1A mGluR5 mGluR5a mGluR5f mGluR5g mGluR5h mGluR5i protein phosphatase 1 regulatory subunit 86 variant b of mGluR5 |
| Molecular Weight(MW) | 132kDa（observed：129kDa) |
| Source | Rabbit |
| Cellular Localization | Cell membrane |
| SwissProt ID | P41594 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using glutamate metabotropic receptor 5 antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),U251(5),A549(6) . 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
