WB
Loading: Supernatant of Escherichia coli lysate Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-6X His Tag antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide of fragment (HHHHHH) His Tag. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | His |
|---|---|
| Alternative Names | His |
| Function | His tag. |
WB
Loading: Supernatant of Escherichia coli lysate Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
