WB
1. Approximately 3 μg Arabidopsis total protein; 2. Arabidopsis transformed with PIF7 gene (C-terminus containing DYKDDDDK) Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-Flag tag antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | FLAG；peptide sequence: DYKDDDDKC |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
| Function | Mouse anti-FLAG antibody recognizes fusion proteins containing the FLAG peptide sequence (DYKDDDDK), including those with the tag at the N-terminus, Met-N-terminus, C-terminus, and internal sites. |
|---|
WB
1. Approximately 3 μg Arabidopsis total protein; 2. Arabidopsis transformed with PIF7 gene (C-terminus containing DYKDDDDK) Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
