WB
Western blot analysis of RUNX2 expression in NIH/3T3 (A), c4 (B), AT3B-1 (C) and PC-3 (D) whole cell lysates.IHC
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.ICC/IF
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymp node tissue showing nuclear staining of subset of cells in non-germinal center.| Product Name | Mouse Monoclonal antibody to RUNX2(F-2) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 294-363 of RUNX2 of mouse origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:100 ICC/IF:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUNX2 |
|---|---|
| Gene Synonyms | CCD AML3 CCD1 CLCD OSF2 CBFA1 OSF-2 PEA2aA PEBP2aA CBF-alpha-1 |
| Gene Full Name | RUNX family transcription factor 2 |
| Gene Summary | This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016] |
| Molecular Weight(MW) | 55 kDa |
WB
Western blot analysis of RUNX2 expression in NIH/3T3 (A), c4 (B), AT3B-1 (C) and PC-3 (D) whole cell lysates.
IHC
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
ICC/IF
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymp node tissue showing nuclear staining of subset of cells in non-germinal center.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:100 ICC/IF:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Also available as TransCruz reagent for Gel Supershift and ChIP applications, sc-390351 X, 200 µg/0.1 ml. |
