ICC/IF
Immunofluorescence staining of formalin-fixed, paraffin-embedded red snapper liver tissue showing 8-OHdG staining in hepatocytes.| Product Name | Mouse Monoclonal antibody to 8-OHdG(E-8) |
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| Antibody Type | Primary Antibodies |
| Immunogen | raised against 8-hydroxy-2'-deoxyguanosine (8-OHdG)-BCP conjugate of synthetic origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM,k |
| Host Species | Mouse |
| Tested Applications | ICC/IF |
| ICC/IF:1:50-1:500 |
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| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Function | DNA or RNA damage can hinder the ability of a cell to carry out its function and can significantly increase the likelihood of tumor formation. One of the causes of damaged DNA and RNA is oxidation of the bases. 8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine (8-OHdG) and 8-hydroxyguanosine are all markers of oxidative damage to RNA and DNA. 8-hydroxy-2'-deoxyguanosine is produced by reactive oxygen and nitrogen species, including hydroxyl radical and peroxynitrite. 8-hydroxyguanine is one of the major base lesions involved in mutagenesis and is caused by ionizing radiation and radiomimetic agents. 8-hydroxyguanosine induces a transversion of G to T in DNA, which may be mutagenic. Markers of DNA and RNA damage are useful research tools when studying the effects of this type of damage. |
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ICC/IF
Immunofluorescence staining of formalin-fixed, paraffin-embedded red snapper liver tissue showing 8-OHdG staining in hepatocytes.| Application Notes | ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 100 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
