WB
Western blot analysis of extracts from PC12 cells, using NMDAR2B Mouse Monoclonal Antibody at 1/1000 dilution. Observed bands:200kD. Exposure for 1MIN with ECL Kit.IHC
NMDAR2B Mouse Monoclonal Antibody at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.ICC/IF
NMDAR2B Mouse Monoclonal Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | NMDAR2B Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human NMDAR2B, corresponding to a region within C-terminal amino acids. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRIN2B |
|---|---|
| Gene Synonyms | NR3 MRD6 NR2B hNR3 DEE27 EIEE27 GluN2B NMDAR2B |
| Gene Full Name | glutamate ionotropic receptor NMDA type subunit 2B |
| Gene Summary | This gene encodes a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. The encoded protein is a subunit of the NMDA receptor ion channel which acts as an agonist binding site for glutamate. The NMDA receptors mediate a slow calcium-permeable component of excitatory synaptic transmission in the central nervous system. The NMDA receptors are heterotetramers of seven genetically encoded, differentially expressed subunits including NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The early expression of this gene in development suggests a role in brain development, circuit formation, synaptic plasticity, and cellular migration and differentiation. Naturally occurring mutations within this gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia. [provided by RefSeq, Aug 2017] |
| Alternative Names | AW490526 EIEE27 Glutamate [NMDA] receptor subunit epsilon 2 Glutamate [NMDA] receptor subunit epsilon-2 Glutamate Receptor Ionotropic N Methyl D Aspartate 2B Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B Glutamate receptor ionotropic NMDA2B Glutamate receptor subunit epsilon 2 Glutamate receptor ionotropic NMDA2B (epsilon 2) GRIN 2B GRIN2B hNR 3 hNR3 MGC142178 MGC142180 MRD6 N methyl D asparate receptor channel subunit epsilon 2 N methyl D aspartate receptor subtype 2B N methyl D aspartate receptor subunit 2B N methyl D aspartate receptor subunit 3 N-methyl D-aspartate receptor subtype 2B N-methyl-D-aspartate receptor subunit 3 NMDA NR2B NMDA R2B NMDAR2B NMDE2 NMDE2_HUMAN NME2 NR2B NR3 |
| Molecular Weight(MW) | 165kDa; 166kD(Calculated). |
| Cellular Localization | Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Late endosome. Lysosome. |
WB
Western blot analysis of extracts from PC12 cells, using NMDAR2B Mouse Monoclonal Antibody at 1/1000 dilution. Observed bands:200kD. Exposure for 1MIN with ECL Kit.
IHC
NMDAR2B Mouse Monoclonal Antibody at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.
ICC/IF
NMDAR2B Mouse Monoclonal Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
