WB
Various lysates were subjected to SDS PAGE followed by western blot with MAP2 antibody at dilution of 1:30000 incubated at room temperature for 1.5 hours.WB
rat brain tissue were subjected to SDS PAGE followed by western blot with MAP2 antibody at dilution of 1:30000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using MAP2 antibody at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-MAP2 (4ug; Detection:1:8000) with mouse brain tissue lysate 1280 ug.IP
IP result of anti-MAP2 (4ug; Detection:1:10000) with SH-SY5Y cells lysate 1240 ug.IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with GFAP antibody, green.IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), GFAP antibody.ICC/IF
Immunofluorescent staining of MAP2 (1:250 dilution) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (RED MAP2; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.FC
1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | MAP2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MAP2 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-FIF-PIHCIPWB |
| WB:1:5000-1:50000 IHC:1:2500-1:10000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IF-P:1:50-1:500 IF-F:1:50-1:500 ICC/IF:1:125-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP2 |
|---|---|
| Gene Synonyms | MAP-2 MAP2A MAP2B MAP2C |
| Gene Full Name | microtubule associated protein 2 |
| Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010] |
| Alternative Names | #REF! |
| Molecular Weight(MW) | 200 kDa(Observed MW: 280 kDa, 70-85 kDa) |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Microtubule. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with MAP2 antibody at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB
rat brain tissue were subjected to SDS PAGE followed by western blot with MAP2 antibody at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using MAP2 antibody at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-MAP2 (4ug; Detection:1:8000) with mouse brain tissue lysate 1280 ug.
IP
IP result of anti-MAP2 (4ug; Detection:1:10000) with SH-SY5Y cells lysate 1240 ug.
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with GFAP antibody, green.
IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), GFAP antibody.
ICC/IF
Immunofluorescent staining of MAP2 (1:250 dilution) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (RED MAP2; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.
FC
1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:5000-1:50000 IHC:1:2500-1:10000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IF-P:1:50-1:500 IF-F:1:50-1:500 ICC/IF:1:125-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
