WB
Western blot analysis of NNT expression in whole cell lysates of Hep G2 (A), Caki-1 (B), A-673 (C), BC3H1 (D), L6 (E) and KNRK (F) using NNT antibody (B-3).WB
Western blot analysis of NNT expression in whole cell lysates of Hep G2 (A), Caki-1 (B), A-673 (C), BC3H1 (D), L6 (E) and KNRK (F) using NNT antibody (B-3).IHC
NNT antibody (B-3):Immunoperoxidase staining of formalin-fixed, paraffin-embedded human myocardial tissue, showing cytoplasmic staining of myocardial cells.ICC/IF
NNT antibody (B-3):Immunofluorescence staining of methanol-fixed HeLa cells, showing mitochondrial localization.ICC/IF
NNT antibody (B-3):Formalin-fixed A-431 cells were subjected to immunofluorescence staining, revealing mitochondrial localization.| Product Name | NNT Antibody(B-3) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 787-1086 mapping at the C-terminus of NNT of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:10000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NNT |
|---|---|
| Gene Synonyms | GCCD4 |
| Gene Full Name | nicotinamide nucleotide transhydrogenase |
| Gene Summary | This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016] |
| Molecular Weight(MW) | 114 kDa |
WB
Western blot analysis of NNT expression in whole cell lysates of Hep G2 (A), Caki-1 (B), A-673 (C), BC3H1 (D), L6 (E) and KNRK (F) using NNT antibody (B-3).
WB
Western blot analysis of NNT expression in whole cell lysates of Hep G2 (A), Caki-1 (B), A-673 (C), BC3H1 (D), L6 (E) and KNRK (F) using NNT antibody (B-3).
IHC
NNT antibody (B-3):Immunoperoxidase staining of formalin-fixed, paraffin-embedded human myocardial tissue, showing cytoplasmic staining of myocardial cells.
ICC/IF
NNT antibody (B-3):Immunofluorescence staining of methanol-fixed HeLa cells, showing mitochondrial localization.
ICC/IF
NNT antibody (B-3):Formalin-fixed A-431 cells were subjected to immunofluorescence staining, revealing mitochondrial localization.| Application Notes | WB:1:1000-1:10000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 50 µg IgG1 light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
