WB
Western blot analysis of ROBLD3 using MCF7 whole cell lysatesIHC
ROBLD3 Antibody at 1/100 staining human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.IHC
ROBLD3 Antibody at 1/100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.| Product Name | ROBLD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human ROBLD3, corresponding to a region within the internal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-3000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LAMTOR2 |
|---|---|
| Gene Synonyms | p14 ENDAP ROBLD3 HSPC003 MAPBPIP MAPKSP1AP Ragulator2 |
| Gene Full Name | late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 |
| Gene Summary | The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
| Alternative Names | ENDAP Endosomal adaptor protein p14 HSPC003 LAMTOR2 Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2 Late endosomal/lysosomal Mp1 interacting protein Late endosomal/lysosomal Mp1-interacting protein LTOR2_HUMAN MAPBPIP MAPKSP1 adaptor protein MAPKSP1AP Mitogen activated protein binding protein interacting protein Mitogen-activated protein-binding protein-interacting protein p14 Ragulator complex protein LAMTOR2 Ragulator2 Roadblock domain containing 3 Roadblock domain containing protein 3 Roadblock domain-containing protein 3 ROBLD 3 RP11 336K24.9 |
| Molecular Weight(MW) | 14 kDa; 14kD(Calculated). |
| Cellular Localization | Late endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lysosome membrane>Peripheral membrane protein>Cytoplasmic side. |
WB
Western blot analysis of ROBLD3 using MCF7 whole cell lysates
IHC
ROBLD3 Antibody at 1/100 staining human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
IHC
ROBLD3 Antibody at 1/100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:1000-3000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
