WB
Western blot analysis of various lysates using Sarm1 Mouse mAb at 1:8000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s