WB
Western blot analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CRKRS/CDK12 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for CRKRS/CDK12 at approximately 205 kDa. The expected band size for CRKRS/CDK12 is at 164 kDa.IHC
IHC analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody. CRKRS/CDK12 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-CRKRS/CDK12 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody. CRKRS/CDK12 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-CRKRS/CDK12 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody and anti-Beta Tubulin antibody. CRKRS/CDK12 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-CRKRS/CDK12 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Fluoro550-conjugated Anti- mouse IgG Secondary Antibody (red) were used as secondary antibody.IP
IP analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody in HepG2 whole cell lysate. Western blot analysis of CRKRS/CDK12 using anti- CRKRS/CDK12 antibody. Lane 1: HepG2 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- CRKRS/CDK12 antibody in HepG2 whole cell lysate, Lane 3: anti- CRKRS/CDK12 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- CRKRS/CDK12 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for CRKRS/CDK12 at approximately 200 kDa. The expected band size for CRKRS/CDK12 is at 164 kDa.FC
Flow Cytometry analysis of HepG2 cells using anti-CRKRS/CDK12 antibody. Overlay histogram showing HepG2 cells stained with A03495-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRKRS/CDK12 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-CRKRS/CDK12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human CDK12 recombinant protein (Position: Q1147-N1439). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK12 |
|---|---|
| Gene Synonyms | CRK7 CRKR CRKRS |
| Gene Full Name | cyclin dependent kinase 12 |
| Gene Summary | Enables RNA polymerase II CTD heptapeptide repeat kinase activity and cyclin binding activity. Involved in several processes, including positive regulation of transcription elongation by RNA polymerase II; protein autophosphorylation; and regulation of MAP kinase activity. Located in nuclear speck. Part of cyclin K-CDK12 complex. Biomarker of gastric adenocarcinoma; hepatocellular carcinoma; and stomach cancer. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | CDC2 related protein kinase 7 CDK12 CDK12/CRKRS CRK7 CRKR CRKRS KIAA0904 |
| Molecular Weight(MW) | 164kDa(Observed MW 205kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CRKRS/CDK12 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for CRKRS/CDK12 at approximately 205 kDa. The expected band size for CRKRS/CDK12 is at 164 kDa.
IHC
IHC analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody. CRKRS/CDK12 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-CRKRS/CDK12 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody. CRKRS/CDK12 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-CRKRS/CDK12 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody and anti-Beta Tubulin antibody. CRKRS/CDK12 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-CRKRS/CDK12 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Fluoro550-conjugated Anti- mouse IgG Secondary Antibody (red) were used as secondary antibody.
IP
IP analysis of CRKRS/CDK12 using anti-CRKRS/CDK12 antibody in HepG2 whole cell lysate. Western blot analysis of CRKRS/CDK12 using anti- CRKRS/CDK12 antibody. Lane 1: HepG2 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- CRKRS/CDK12 antibody in HepG2 whole cell lysate, Lane 3: anti- CRKRS/CDK12 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- CRKRS/CDK12 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for CRKRS/CDK12 at approximately 200 kDa. The expected band size for CRKRS/CDK12 is at 164 kDa.
FC
Flow Cytometry analysis of HepG2 cells using anti-CRKRS/CDK12 antibody. Overlay histogram showing HepG2 cells stained with A03495-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRKRS/CDK12 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
