WB
HL-60 cells were subjected to SDS PAGE followed by western blot with (GZMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using (GZMB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using (Granzyme B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Granzyme B antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Granzyme B antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).| Product Name | Granzyme B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human GZMB protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIF-FIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:500 IF-P:1:50-1:500 IF-FRO: 1:50-1:500 IF/ICC: 1:200-1:800 |
|
| Species Reactivity | HumanMouse |
| Purification | Affinity purified |
| Gene Symbol | GZMB |
|---|---|
| Gene Synonyms | C11 HLP CCPI CGL1 CSPB SECT CGL-1 CSP-B CTLA1 CTSGL1 |
| Gene Full Name | granzyme B |
| Gene Summary | This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016] |
| Alternative Names | GZMB Cathepsin G-like 1 CCPI CGL 1 CGL1 |
| Molecular Weight(MW) | 28 kDa(Observed band size:33 kDa) |
| Cellular Localization | Lysosome,Secreted |
WB
HL-60 cells were subjected to SDS PAGE followed by western blot with (GZMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using (GZMB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using (Granzyme B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Granzyme B antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Granzyme B antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 IF-P:1:50-1:500 IF-FRO: 1:50-1:500 IF/ICC: 1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | tore at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
