WB
Western blot analysis of ATRX on different lysates with Rabbit anti-ATRX antibody at 1/2,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cells) cell lysate Lane 2: U-2 OS (Human osteosarcoma cells) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 srconds; ECL: K1801 U-2 OS is a negative control (PMID: 22829774). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody:1/2,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureICC/IF
Application: Immunocytochemistry (IF-cell) Species: Human Sample: SH-SY5Y (Human Neuroblastoma Cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody:1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488), 45 minutes at room temperature. Counterstain: Beta tubulin (red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).FC
Application: Flow Cytometry (Intra) Species: Human Sample: SH-SY5Y (Human neuroblastoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: (1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488), 15 minutes at room temperature.FC
Application: Flow Cytometry (Intra) Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: (1 μg/mL) (Red) compared with Rabbit IgG Isotype Control ( Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488), 15 minutes at room temperature.| Product Name | ATRX Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human ATRX aa 2,193-2,492. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:2000 ICC/IF:1:100 FC:1 μg/mL |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATRX |
|---|---|
| Gene Synonyms | JMS XH2 XNP MRX52 RAD54 RAD54L ZNF-HX |
| Gene Full Name | ATRX chromatin remodeler |
| Gene Summary | The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2017] |
| Alternative Names | Alpha thalassemia/mental retardation syndrome X linked homolog antibody ATP dependent helicase ATRX antibody ATP-dependent helicase ATRX antibody ATR2 antibody Atrx antibody ATRX_HUMAN antibody DNA dependent ATPase and helicase antibody Helicase 2 X linked antibody MGC2094 antibody MRXHF1 antibody Alpha thalassemia/mental retardation syndrome X linked homolog antibody ATP dependent helicase ATRX antibody ATP-dependent helicase ATRX antibody ATR2 antibody Atrx antibody ATRX_HUMAN antibody DNA dependent ATPase and helicase antibody Helicase 2 X linked antibody MGC2094 antibody MRXHF1 antibody RAD54 antibody RAD54L antibody SFM1 antibody SHS antibody Transcriptional regulator ATRX antibody X linked helicase II antibody X linked nuclear protein antibody X-linked helicase II antibody X-linked nuclear protein antibody XH2 antibody XNP antibody Znf HX antibody Znf-HX antibody |
| Molecular Weight(MW) | 283 kDa（Observed band size: 400 kDa） |
| Cellular Localization | Nucleus, Chromosome, telomere, PML body. |
WB
Western blot analysis of ATRX on different lysates with Rabbit anti-ATRX antibody at 1/2,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cells) cell lysate Lane 2: U-2 OS (Human osteosarcoma cells) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 srconds; ECL: K1801 U-2 OS is a negative control (PMID: 22829774). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody:1/2,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Human Sample: SH-SY5Y (Human Neuroblastoma Cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody:1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488), 45 minutes at room temperature. Counterstain: Beta tubulin (red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
FC
Application: Flow Cytometry (Intra) Species: Human Sample: SH-SY5Y (Human neuroblastoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: (1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488), 15 minutes at room temperature.
FC
Application: Flow Cytometry (Intra) Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: (1 μg/mL) (Red) compared with Rabbit IgG Isotype Control ( Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488), 15 minutes at room temperature.| Application Notes | WB:1:2000 ICC/IF:1:100 FC:1 μg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
