WB
Direct fluorescentwesternblotanalysisofABCA4expression inmouseeyetissueextract.IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fetal eye tissue showing membrane and cytoplasmic staining of cells in retina. Notice the intense staining of the rod outer segment layer.| Product Name | Mouse Monoclonal antibody to ABCA4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Firefly Luciferase Antibody is a mouse monoclonal IgG2a κ antibody against luciferase (also referred to as anti-firefly luciferase antibody). It enables the detection of luciferase protein derived from Photinus pyralis via Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF) and Enzyme-Linked Immunosorbent Assay (ELISA).
This anti-luciferase antibody is available in both unconjugated form and a variety of conjugated formats, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates.
Luciferase isolated from Photinus pyralis, a common North American firefly, is one of the most extensively studied enzymes that catalyze light production in bioluminescent organisms. As a member of the ATP-dependent AMP-binding enzyme family, it emits green light at a wavelength of 562 nm.
The chemical reaction catalyzed by luciferase is as follows: Photinus luciferin + O₂ + ATP → Oxidized Photinus luciferin + CO₂ + AMP + Pyrophosphate + Light |
| Immunogen | raised against the 220 kDa ABCA4 protein of bovine origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCA4 |
|---|---|
| Gene Synonyms | FFM RMP ABCR RP19 STGD ABC10 ARMD2 CORD3 STGD1 |
| Gene Full Name | ATP binding cassette subfamily A member 4 |
| Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, and the gene product mediates transport of an essental molecule, all-trans-retinal aldehyde (atRAL), across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease, a form of juvenile-onset macular degeneration. Mutations in this gene are also associated with retinitis pigmentosa-19, cone-rod dystrophy type 3, early-onset severe retinal dystrophy, fundus flavimaculatus, and macular degeneration age-related 2. [provided by RefSeq, Sep 2019] |
| Molecular Weight(MW) | 220 kDa. |
WB
Direct fluorescentwesternblotanalysisofABCA4expression inmouseeyetissueextract.
IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fetal eye tissue showing membrane and cytoplasmic staining of cells in retina. Notice the intense staining of the rod outer segment layer.| Application Notes | WB:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
