WB
Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody at 1/1,000 dilution. Lane 1: U937 cell lysate Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37-60 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | uPA Receptor Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human uPA Receptor aa 51-250/335. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PLAUR |
|---|---|
| Gene Synonyms | CD87 UPAR URKR U-PAR |
| Gene Full Name | plasminogen activator, urokinase receptor |
| Gene Summary | This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008] |
| Alternative Names | CD 87 antibody CD87 antibody CD87 antigen antibody MO 3 antibody MO3 antibody Monocyte activation antigen Mo3 antibody Plasminogen activator receptor urokinase antibody Plasminogen activator urokinase receptor antibody PLAUR antibody U PAR antibody CD 87 antibody CD87 antibody CD87 antigen antibody MO 3 antibody MO3 antibody Monocyte activation antigen Mo3 antibody Plasminogen activator receptor urokinase antibody Plasminogen activator urokinase receptor antibody PLAUR antibody U PAR antibody u plasminogen activator receptor antibody U-PAR antibody u-plasminogen activator receptor form 2 antibody UPA receptor antibody uPAR antibody UPAR_HUMAN antibody Urinary plasminogen activator receptor antibody URKR antibody Urokinase plasminogen activator receptor antibody Urokinase plasminogen activator surface receptor antibody urokinase-type plasminogen activator (uPA) receptor antibody |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cell projection,Membrane,Secreted |
WB
Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody at 1/1,000 dilution. Lane 1: U937 cell lysate Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37-60 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
