WB
Western blot analysis of ADAM15 on different lysates with Rabbit anti-ADAM15 antibody at 1/1,000 dilution. Lane 1: SW480 cell lysate (15 µg/Lane) Lane 2: HUVEC cell lysate (15 µg/Lane) Lane 3: A431 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: Mouse colon tissue lysate (20 µg/Lane) Lane 6: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ADAM15 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ADAM15 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human ADAM15 aa 250-350 (extracellular). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ADAM15 |
|---|---|
| Gene Synonyms | MDC15 |
| Gene Full Name | ADAM metallopeptidase domain 15 |
| Gene Summary | The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Alternative Names | A disintegrin and metalloproteinase domain 15 (metargidin) antibody A disintegrin and metalloproteinase domain 15 antibody ADA15_HUMAN antibody ADAM 15 antibody ADAM metallopeptidase domain 15 antibody Adam15 antibody and cysteine-rich protein 15 antibody Disintegrin and metalloproteinase domain-containing protein 15 antibody disintegrin-like antibody EC 3.4.24. antibody A disintegrin and metalloproteinase domain 15 (metargidin) antibody A disintegrin and metalloproteinase domain 15 antibody ADA15_HUMAN antibody ADAM 15 antibody ADAM metallopeptidase domain 15 antibody Adam15 antibody and cysteine-rich protein 15 antibody Disintegrin and metalloproteinase domain-containing protein 15 antibody disintegrin-like antibody EC 3.4.24. antibody MDC 15 antibody MDC-15 antibody MDC15 antibody Metalloprotease RGD disintegrin protein antibody Metalloproteinase like disintegrin like and cysteine rich protein 15 antibody Metalloproteinase-like antibody Metargidin antibody |
| Molecular Weight(MW) | 93 kDa |
| Cellular Localization | Cell junction,Cell projection,Cilium,Cytoplasmic vesicle,Flagellum,Membrane |
WB
Western blot analysis of ADAM15 on different lysates with Rabbit anti-ADAM15 antibody at 1/1,000 dilution. Lane 1: SW480 cell lysate (15 µg/Lane) Lane 2: HUVEC cell lysate (15 µg/Lane) Lane 3: A431 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: Mouse colon tissue lysate (20 µg/Lane) Lane 6: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ADAM15 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
