WB
Y79 cells were subjected to SDS PAGE followed by western blot with(CRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-CRX with Y79 cells lysate 1600 ug.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CRX antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).| Product Name | CRX Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CRX protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC: 1:2000-1:8000 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | CRX |
|---|---|
| Gene Synonyms | CRD LCA7 OTX3 CORD2 |
| Gene Full Name | cone-rod homeobox |
| Gene Summary | The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | cone rod homeobox Cone-rod homeobox protein CORD2 CRD |
| Molecular Weight(MW) | 37 kDa(Observed band size:37 kDa) |
| Cellular Localization | Nucleus. |
WB
Y79 cells were subjected to SDS PAGE followed by western blot with(CRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-CRX with Y79 cells lysate 1600 ug.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CRX antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).| Application Notes | WB:1:500-1:1000 IHC: 1:2000-1:8000 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
