WB
Western blot analysis of anti-NBR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NBR1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for NBR1 at approximately 140 kDa. The expected band size for NBR1 is at 107,140 kDa.ICC/IF
ICC/IF analysis of NBR1 using anti-NBR1 antibody and anti-Beta Tubulin antibody. NBR1 was detected in an immunocytochemical section of A549 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.FC
Flow Cytometry analysis of MCF-7 cells using anti-NBR1 antibody. Overlay histogram showing MCF-7 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NBR1 Antibody. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Anti-NBR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NBR1 recombinant protein (Position: M1-H665). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 ELISA:1:100-1000 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NBR1 |
|---|---|
| Gene Synonyms | IAI3B M17S2 MIG19 1A1-3B |
| Gene Full Name | NBR1 autophagy cargo receptor |
| Gene Summary | The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Alternative Names | 1A1 3B 1A13B KIAA0049 M17S2 MIG19 NBR1 neighbor of BRCA1 gene 1 Next to BRCA1 gene 1 protein Protein 1A1 3B |
| Molecular Weight(MW) | 107 kDa(Observed band size:140 kDa) |
| Cellular Localization | Cytoplasm,Cytoplasmic vesicle,Lysosome |
WB
Western blot analysis of anti-NBR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NBR1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for NBR1 at approximately 140 kDa. The expected band size for NBR1 is at 107,140 kDa.
ICC/IF
ICC/IF analysis of NBR1 using anti-NBR1 antibody and anti-Beta Tubulin antibody. NBR1 was detected in an immunocytochemical section of A549 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
FC
Flow Cytometry analysis of MCF-7 cells using anti-NBR1 antibody. Overlay histogram showing MCF-7 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NBR1 Antibody. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 ELISA:1:100-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
