IHC
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,NBR1 Mouse Monoclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NBR1 Mouse Monoclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of rat-spleen tissue. 1,NBR1 Mouse Monoclonal Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Product Name | NBR1 Mouse mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Protein of NBR1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| ICC/IF:1:50-200 IHC:1:50-300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NBR1 |
|---|---|
| Gene Synonyms | IAI3B M17S2 MIG19 1A1-3B |
| Gene Full Name | NBR1 autophagy cargo receptor |
| Gene Summary | The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Alternative Names | Next to BRCA1 gene 1 protein Cell migration-inducing gene 19 protein Membrane component chromosome 17 surface marker 2 Neighbor of BRCA1 gene 1 protein Protein 1A1-3B |
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Cytoplasm . Cytoplasmic vesicle , autophagosome . Lysosome . Cytoplasm , myofibril , sarcomere , M line . In cardiac muscles localizes to the sarcomeric M line (By similarity) . Is targeted to lysosomes for degradation (PubMed:19250911). |
IHC
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,NBR1 Mouse Monoclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NBR1 Mouse Monoclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of rat-spleen tissue. 1,NBR1 Mouse Monoclonal Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Application Notes | ICC/IF:1:50-200 IHC:1:50-300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
