WB
Western blot analysis of various lysates using SMC4 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using SMC4 Rabbit mAb at dilution of 1:100 (40x lens) Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | SMC4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:500 - 1:2000 ICC/IF:1:50 - 1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMC4 |
|---|---|
| Gene Synonyms | CAPC CAP-C SMC-4 SMC4L1 |
| Gene Full Name | structural maintenance of chromosomes 4 |
| Gene Summary | This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2. [provided by RefSeq, Jun 2016] |
| Alternative Names | CAPC CAP-C SMC-4 SMC4L1 SMC4 |
| Molecular Weight(MW) | 147kDa(Observed band size:180kDa) |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus |
WB
Western blot analysis of various lysates using SMC4 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using SMC4 Rabbit mAb at dilution of 1:100 (40x lens) Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500 - 1:2000 ICC/IF:1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
