ICC/IF
Immunofluorescence analysis of HeLa cells labelling SMC4. The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16211 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488. Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Product Name | SMC4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of human SMC4 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB: 1:1000-1:5000 ICC/IF: 1:100 FC: 1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMC4 |
|---|---|
| Gene Synonyms | CAPC CAP-C SMC-4 SMC4L1 |
| Gene Full Name | structural maintenance of chromosomes 4 |
| Gene Summary | This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2. [provided by RefSeq, Jun 2016] |
| Alternative Names | CAPC CAP-C SMC-4 SMC4L1 |
| Molecular Weight(MW) | 147 kDa(Observed band size:180 kDa) |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus |
ICC/IF
Immunofluorescence analysis of HeLa cells labelling SMC4. The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16211 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488. Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Application Notes | WB: 1:1000-1:5000 ICC/IF: 1:100 FC: 1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. |
| Storage Buffer | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
