WB
Western blot analysis of GGT1 on different lysates with Rabbit anti-GGT1 antibody at 1/1000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane), Lane 2: A549 cell lysate (20 µg/Lane), Lane 3: Human liver tissue lysate (40 µg/Lane), Lane 4: Human kidney tissue lysate (40 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GGT1 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | GGT1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within Human GGT1 aa 1-100 / 569. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GGT1 |
|---|---|
| Gene Synonyms | GGT GTG GGTD CD224 GGT 1 D22S672 D22S732 |
| Gene Full Name | gamma-glutamyltransferase 1 |
| Gene Summary | The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014] |
| Alternative Names | CD224 antibody D22S672 antibody D22S732 antibody Gamma glutamyl transpeptidase antibody Gamma glutamyltransferase 1 antibody Gamma glutamyltranspeptidase 1 antibody Gamma-glutamyltransferase 1 antibody Gamma-glutamyltranspeptidase 1 light chain antibody GGT 1 antibody GGT antibody CD224 antibody D22S672 antibody D22S732 antibody Gamma glutamyl transpeptidase antibody Gamma glutamyltransferase 1 antibody Gamma glutamyltranspeptidase 1 antibody Gamma-glutamyltransferase 1 antibody Gamma-glutamyltranspeptidase 1 light chain antibody GGT 1 antibody GGT antibody GGT1 antibody GGT1_HUMAN antibody Glutamyl transpeptidase antibody Glutathione hydrolase 1 antibody GTG antibody Leukotriene C4 hydrolase antibody MGC96892 antibody MGC96904 antibody MGC96963 antibody OTTHUMP00000028921 antibody OTTHUMP00000197959 antibody |
| Molecular Weight(MW) | 61kDa(Observed band size: 60-70kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of GGT1 on different lysates with Rabbit anti-GGT1 antibody at 1/1000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane), Lane 2: A549 cell lysate (20 µg/Lane), Lane 3: Human liver tissue lysate (40 µg/Lane), Lane 4: Human kidney tissue lysate (40 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GGT1 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
