WB
Western blot analysis of lysates from Mouse skeletal muscle, using MYH4 Rabbit pAb at 1:400dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.WB
Western blot analysis of various lysates, using MYH4 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart using MYH4 RabbitpAb at dilution of 1:100 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using MYH4 Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.IF-P
Immunofluorescence analysis of paraffinembedded rat skeletal muscle using MYH4Rabbit pAb at dilution of 1:100(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI fornuclear staining.| Product Name | MYH4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:5000 IF-P:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH4 |
|---|---|
| Gene Synonyms | MYH2B MyHC-2B MyHC-IIb |
| Gene Full Name | myosin heavy chain 4 |
| Gene Summary | Enables double-stranded RNA binding activity. Involved in muscle contraction. Located in myofibril. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | MYH2B MyHC-2B MyHC-IIb MYH4 |
| Molecular Weight(MW) | 223kDa(Observed band size:250kDa) |
| Cellular Localization | Cytoplasm, myofibril. |
WB
Western blot analysis of lysates from Mouse skeletal muscle, using MYH4 Rabbit pAb at 1:400dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
WB
Western blot analysis of various lysates, using MYH4 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart using MYH4 RabbitpAb at dilution of 1:100 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using MYH4 Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IF-P
Immunofluorescence analysis of paraffinembedded rat skeletal muscle using MYH4Rabbit pAb at dilution of 1:100(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI fornuclear staining.| Application Notes | WB:1:500-1:5000 IF-P:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
