WB
Western blot analysis of various lysates using MYH4 Rabbit mAb at 1:5000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): Mouse brain,Rat brainExposure time: 5s.IHC
Immunohistochemistry analysis of paraffinembedded Human heart tissue using MYH4Rabbit mAb at a dilution of 1:500(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.| Product Name | MYH4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:5000-1:20000 IHC:1:400-1:1600 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH4 |
|---|---|
| Gene Synonyms | MYH2B MyHC-2B MyHC-IIb |
| Gene Full Name | myosin heavy chain 4 |
| Gene Summary | Enables double-stranded RNA binding activity. Involved in muscle contraction. Located in myofibril. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | MYH2B MyHC-2B MyHC-IIb |
| Molecular Weight(MW) | 223kDa(Observed band size:250kDa) |
| Cellular Localization | Cytoplasm, myofibril. |
WB
Western blot analysis of various lysates using MYH4 Rabbit mAb at 1:5000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): Mouse brain,Rat brainExposure time: 5s.
IHC
Immunohistochemistry analysis of paraffinembedded Human heart tissue using MYH4Rabbit mAb at a dilution of 1:500(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.| Application Notes | WB:1:5000-1:20000 IHC:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
