WB
Western blot analysis of lysates from Rat lung, using IKZF3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.WB
Western blot analysis of lysates from Jurkat cells, using IKZF3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.IF-P
Confocal imaging of paraffin-embeddedHuman spleen tissue using IKZF3 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 40x. Perform high pressureantigen retrieval with 0.01 M citrate buffer(pH 6.0) prior to IF staining.IHC
Immunohistochemistry analysis of paraffinembedded Human appendix using IKZF3Rabbit mAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M Tris/EDTA Buffer (pH9.0) prior to IHC staining.| Product Name | IKZF3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKZF3 |
|---|---|
| Gene Synonyms | AIO IMD84 AIOLOS ZNFN1A3 |
| Gene Full Name | IKAROS family zinc finger 3 |
| Gene Summary | This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012] |
| Alternative Names | AIO IMD84 AIOLOS ZNFN1A3 IKZF3 |
| Molecular Weight(MW) | 58kDa(Observed band size:50-70kDa) |
| Cellular Localization | cytoplasm, cytosol, nucleoplasm, nucleus. |
WB
Western blot analysis of lysates from Rat lung, using IKZF3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
WB
Western blot analysis of lysates from Jurkat cells, using IKZF3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
IF-P
Confocal imaging of paraffin-embeddedHuman spleen tissue using IKZF3 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 40x. Perform high pressureantigen retrieval with 0.01 M citrate buffer(pH 6.0) prior to IF staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human appendix using IKZF3Rabbit mAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M Tris/EDTA Buffer (pH9.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
