WB
Western blot analysis of various lysates using IKZF3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.WB
Western blot analysis of various lysates using IKZF3 Rabbit mAb at 1:1000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): U-937.Exposure time: 45 s.IHC
Immunohistochemistry analysis of paraffinembedded Human spleen tissue using IKZF3Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen tissue using IKZF3Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue using IKZF3Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.IF-P
Immunofluorescence analysis of paraffinembedded rat spleen using IKZF3 Rabbit mAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.IP
Immunoprecipitation analysis of 300 μg extracts of Raji cells using 3 μg IKZF3 antibody. Westernblot was performed from the immunoprecipitate using IKZF3 antibody (A8614) at a dilution of 1:500.| Product Name | IKZF3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIPWB |
| WB:1:500-1:1000 IHC:1:200-1:800 IF-P:1:50-1:200 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKZF3 |
|---|---|
| Gene Synonyms | AIO IMD84 AIOLOS ZNFN1A3 |
| Gene Full Name | IKAROS family zinc finger 3 |
| Gene Summary | This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012] |
| Alternative Names | AIO IMD84 AIOLOS ZNFN1A3 IKZF3 |
| Molecular Weight(MW) | 58kDa(Observed band size:50-70kDa) |
| Cellular Localization | Cytoplasm, Cytosol, Nucleoplasm, Nucleus. |
WB
Western blot analysis of various lysates using IKZF3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
WB
Western blot analysis of various lysates using IKZF3 Rabbit mAb at 1:1000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): U-937.Exposure time: 45 s.
IHC
Immunohistochemistry analysis of paraffinembedded Human spleen tissue using IKZF3Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen tissue using IKZF3Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue using IKZF3Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.
IF-P
Immunofluorescence analysis of paraffinembedded rat spleen using IKZF3 Rabbit mAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
IP
Immunoprecipitation analysis of 300 μg extracts of Raji cells using 3 μg IKZF3 antibody. Westernblot was performed from the immunoprecipitate using IKZF3 antibody (A8614) at a dilution of 1:500.| Application Notes | WB:1:500-1:1000 IHC:1:200-1:800 IF-P:1:50-1:200 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
