WB
Western blot analysis of various lysates using Myelin Protein Zero (MPZ) Rabbit mAb at1:19000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.| Product Name | Myelin Protein Zero (MPZ) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:20000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MPZ |
|---|---|
| Gene Synonyms | P0 CHM DSS MPP CHN2 CMT1 CMT1B CMT2I CMT2J CMT4E CMTDI3 CMTDID HMSNIB |
| Gene Full Name | myelin protein zero |
| Gene Summary | This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in this gene are associated with autosomal dominant form of Charcot-Marie-Tooth disease type 1 (CMT1B) and other polyneuropathies, such as Dejerine-Sottas syndrome (DSS) and congenital hypomyelinating neuropathy (CHN). A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2015] |
| Alternative Names | P0 CHM DSS MPP CHN2 CMT1 CMT1B CMT2I CMT2J CMT4E CMTDI3 CMTDID HMSNIB Myelin Protein Zero (MPZ) |
| Molecular Weight(MW) | 28kDa(Observed band size:28kDa) |
| Cellular Localization | Cell membrane, Myelin membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using Myelin Protein Zero (MPZ) Rabbit mAb at1:19000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.| Application Notes | WB:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
