WB
Western blot analysis of P0/MPZ using anti-P0/MPZ . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P0/MPZ antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for P0/MPZ at approximately 22 kDa. The expected band size for P0/MPZ is at 22 kDa.IHC
IHC analysis of P0/MPZ using anti-P0/MPZ .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of P0/MPZ using anti-P0/MPZ .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF
IF analysis of P0/MPZ using anti-P0/MPZ antibody .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .IF
IF analysis of P0/MPZ using anti-P0/MPZ antibody .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .| Product Name | Anti-Zebrafish P0/MPZ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish P0/MPZ recombinant protein (Position: Q18-P206). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | myelin protein zero |
|---|---|
| Target | MPZ |
| Alternative Names | CHM CMT1 CMT1B CMT2I CMT2J CMT4E CMTDI3 DSS HMSNIB MPP MPZ MPZ / P0 Myelin peripheral protein Myelin protein P0 myelin protein zero P0 |
| Molecular Weight(MW) | 22 kDa |
| SwissProt ID | A0A0R4IS04 |
|---|
WB
Western blot analysis of P0/MPZ using anti-P0/MPZ . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P0/MPZ antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for P0/MPZ at approximately 22 kDa. The expected band size for P0/MPZ is at 22 kDa.
IHC
IHC analysis of P0/MPZ using anti-P0/MPZ .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of P0/MPZ using anti-P0/MPZ .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF
IF analysis of P0/MPZ using anti-P0/MPZ antibody .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .
IF
IF analysis of P0/MPZ using anti-P0/MPZ antibody .P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-P0/MPZ Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
