WB
Western blot analysis of lysates from 293F cells, using ADAR1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.ICC/IF
Immunofluorescence analysis of HepG2 cellsusing ADAR1 Rabbit pAb at dilutionof 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon using ADAR1 RabbitpAb at dilution of 1:50 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Product Name | ADAR1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:400-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAR |
|---|---|
| Gene Synonyms | DSH AGS6 G1P1 IFI4 P136 ADAR1 DRADA DSRAD IFI-4 K88DSRBP |
| Gene Full Name | adenosine deaminase RNA specific |
| Gene Summary | This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| Alternative Names | DSH AGS6 G1P1 IFI4 P136 ADAR1 DRADA DSRAD IFI-4 K88DSRBP |
| Molecular Weight(MW) | 136kDa(Observed band size:110-150kDa) |
| Cellular Localization | Cytoplasm, Nucleus, Nucleus, nucleolus. |
WB
Western blot analysis of lysates from 293F cells, using ADAR1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of HepG2 cellsusing ADAR1 Rabbit pAb at dilutionof 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon using ADAR1 RabbitpAb at dilution of 1:50 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:400-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
