WB
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ALOX15 usingALOX15 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using ALOX15 Rabbit pAb atdilution of 1:50 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L)at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing ALOX15 Rabbit pAb at dilutionof 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of MCF7 cellsusing ALOX15 Rabbit pAb at a dilution of 1:150 (40x lens). Secondaryantibody:Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPIfor nuclear staining.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing ALOX15 Rabbit pAb at a dilution of 1:150 (40x lens). Secondaryantibody:Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPIfor nuclear staining.| Product Name | ALOX15 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALOX15 |
|---|---|
| Gene Synonyms | LOG15 12-LOX 15-LOX 15-LOX-1 |
| Gene Full Name | arachidonate 15-lipoxygenase |
| Gene Summary | This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 75kDa(Observed band size:75kDa) |
| Cellular Localization | Cell membrane, Cytoplasm, Lipid droplet, Peripheral membrane protein, cytosol. |
WB
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ALOX15 usingALOX15 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using ALOX15 Rabbit pAb atdilution of 1:50 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L)at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing ALOX15 Rabbit pAb at dilutionof 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of MCF7 cellsusing ALOX15 Rabbit pAb at a dilution of 1:150 (40x lens). Secondaryantibody:Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPIfor nuclear staining.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing ALOX15 Rabbit pAb at a dilution of 1:150 (40x lens). Secondaryantibody:Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPIfor nuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
