WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKA α/β/γ (Phospho Thr197) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela starved of serum overnight and then treated with 20% fetal bovine serum and Calyculin A(100 nM) for 15 minutes.IHC
Human hepatocellular carcinoma was stained with anti-PKA α/β/γ (Phospho Thr197) Rabbit antibody.| Product Name | PKA α/β/γ (Phospho Thr197) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgE |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKACA |
|---|---|
| Gene Synonyms | CAFD1 PKACA PPNAD4 |
| Gene Full Name | protein kinase cAMP-activated catalytic subunit alpha |
| Gene Summary | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015] |
| Alternative Names | PRKACA PKACA cAMP-dependent protein kinase catalytic subunit alpha PKA C-alpha PRKACB cAMP-dependent protein kinase catalytic subunit beta PKA C-beta PRKACG cAMP-dependent protein kinase catalytic subunit gamma PKA C-gamma |
| Molecular Weight(MW) | 41kDa(Observed MW 41kDa) |
| Cellular Localization | Cytoplasm. Cell membrane. Nucleus . Mitochondrion . Membrane. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKA α/β/γ (Phospho Thr197) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela starved of serum overnight and then treated with 20% fetal bovine serum and Calyculin A(100 nM) for 15 minutes.
IHC
Human hepatocellular carcinoma was stained with anti-PKA α/β/γ (Phospho Thr197) Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
