WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VEGF Receptor 2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat heart Lane 2: HT-1080.WB
Western blot analysis of lysates from SK-OV3 cells, using VEGFR2 Antibody. The lane on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of Human placenta.IHC
Human placenta was stained with anti-VEGF Receptor 2 rabbit antibody.| Product Name | Flk-1/VEGFR2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:1000-1:4000 ICC/IF:1:200-1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KDR |
|---|---|
| Gene Synonyms | FLK1 CD309 VEGFR VEGFR2 |
| Gene Full Name | kinase insert domain receptor |
| Gene Summary | Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009] |
| Alternative Names | KDR FLK1 VEGFR2 Vascular endothelial growth factor receptor 2 VEGFR-2 Fetal liver kinase 1 FLK-1 Kinase insert domain receptor KDR Protein-tyrosine kinase receptor flk-1 CD antigen CD309 |
| Molecular Weight(MW) | 151kDa(Observed MW 230kDa) |
| Cellular Localization | Cell junction.Endoplasmic reticulum.Cell membrane. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VEGF Receptor 2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat heart Lane 2: HT-1080.
WB
Western blot analysis of lysates from SK-OV3 cells, using VEGFR2 Antibody. The lane on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of Human placenta.
IHC
Human placenta was stained with anti-VEGF Receptor 2 rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:1000-1:4000 ICC/IF:1:200-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
